更新時(shí)間:2016-06-11
卵磷脂吐溫80營養(yǎng)瓊脂,年中放價(jià)銷售了,低價(jià)、*、優(yōu)惠價(jià)供應(yīng),微生物培養(yǎng)基折扣價(jià)提供,BD培養(yǎng)基、Gibco培養(yǎng)基、Amresco培養(yǎng)基、OXOID培養(yǎng)基等國內(nèi)外培養(yǎng)基,我們傾情提供。
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卵磷脂吐溫80營養(yǎng)瓊脂 | Lecithin Tween 80 Nutrient Agar | 250g | 用于化妝品細(xì)菌總數(shù)測定(GB標(biāo)準(zhǔn)) |
用途:用于化妝品細(xì)菌總數(shù)測定(GB標(biāo)準(zhǔn))
卵磷脂吐溫80營養(yǎng)瓊脂配方:(g/L) | |||||||||||||||||||||||||||||||||
蛋白胨 氯化鈉 牛肉粉 卵磷脂 吐溫80 瓊脂 pH值7.2 ± 0.1 | 20.0 5.0 3.0 1.0 7.0 12.0 25℃ | ||||||||||||||||||||||||||||||||
原理: | |||||||||||||||||||||||||||||||||
蛋白胨和牛肉浸粉提供碳氮源、維生素和生長因子;氯化鈉維持均衡的滲透壓;卵磷脂和吐溫80能中和防腐劑,并能起到在乳濁液中分散物質(zhì)的功能,卵磷脂能中和季銨鹽,吐溫80可中和酚、六氯酚、福爾馬林,兩者結(jié)合可中和乙醇;瓊脂是培養(yǎng)基的凝固劑;大多數(shù)細(xì)菌能還原TTC,使之形成易于辨別的紅色菌落,TTC還能減緩某些細(xì)菌的蔓延生長。 | |||||||||||||||||||||||||||||||||
用法: | |||||||||||||||||||||||||||||||||
稱取本品 48.0g,加熱溶解于 1000ml 蒸餾水中,分裝, 121℃高壓滅菌 15 分鐘, 備用。 | |||||||||||||||||||||||||||||||||
質(zhì)量控制: | |||||||||||||||||||||||||||||||||
在36±1℃培養(yǎng)18-24小時(shí)。
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